Genetic characterization of dengue virus type 3 isolates in the State of Rio de Janeiro, 2001
Open Access
- 1 August 2002
- journal article
- Published by FapUNIFESP (SciELO) in Brazilian Journal of Medical and Biological Research
- Vol. 35 (8) , 869-872
- https://doi.org/10.1590/s0100-879x2002000800002
Abstract
The genetic characterization of dengue virus type 3 (DEN-3) strains isolated from autochthonous cases in the State of Rio de Janeiro, Brazil, in 2001 is presented. Restriction site-specific (RSS)-PCR performed on 22 strains classified the Brazilian DEN-3 viruses as subtype C, a subtype that contains viruses from Sri Lanka, India, Africa and recent isolates from Central America. Nucleic acid sequencing (positions 278 to 2550) of one DEN-3 strain confirmed the origin of these strains, since genotype III - classified by sequencing - and RSS-PCR subtype C are correlated. This genetic subtype has been associated with hemorrhagic dengue epidemics and the information provided here could be useful to implement appropriate prevention and control measures.Keywords
This publication has 12 references indexed in Scilit:
- Dengue virus type 3 in Rio de Janeiro, BrazilMemórias do Instituto Oswaldo Cruz, 2001
- First isolation of dengue 3 in Brazil from an imported caseRevista do Instituto de Medicina Tropical de São Paulo, 2001
- The Brazilian flavivirusesMicrobes and Infection, 2000
- Dengue Virus (Serotype 3) Circulation in Endemic Countries and Its Reappearance in AmericaArchives of Medical Research, 2000
- Dengue in the State of Rio de Janeiro, Brazil, 1986-1998Memórias do Instituto Oswaldo Cruz, 1999
- Rapid Subtyping of Dengue Viruses by Restriction Site-Specific (RSS)–PCRVirology, 1999
- Dengue in Brazil II: laboratory aspects and perspectivesVIRUS Reviews & Research, 1996
- Molecular evolution and epidemiology of dengue-3 virusesJournal of General Virology, 1994
- Isolation of dengue virus type 2 in Rio de JaneiroMemórias do Instituto Oswaldo Cruz, 1990
- FlaviviridaeIntervirology, 1985