Antigen-Binding Activity of 6 S Subunits of β 2 -Macroglobulin Antibody
- 16 October 1964
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 146 (3642) , 404-405
- https://doi.org/10.1126/science.146.3642.404
Abstract
Direct evidence is provided for the antigen binding activity of the 6 S subunits formed by reduction and alkylation of rabbit β 2 -macroglobulin antibody. Binding activity of the subunits was clearly demonstrated by radioimmunoelectrophoresis with a preparation of purified rabbit antibody against the p -azobenzenearsonate group, which contained 40 percent of β 2M -antibody. The precipitate arc formed between the subunits and sheep antiserum against rabbit macroglobulin was shown to bind radioactive antigen specifically.Keywords
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