“Reversed-phase” partition chromatography with water/alcohol solvents and Zipax® column packings with nonpolar stationary phases permits a rapid and reliable method for the analysis of the fat-soluble vitamins (vitamins A, E, D and K) and their esters. The chromatographic conditions needed to separate the vitamins are developed, and detector sensitivity, linear dynamic range, and the precision of the technique in terms of retention time and peak area reproducibility are reported. Some application problems in fat-soluble vitamin analysis are discussed.