Characterization of trehalose tetraester produced by a waste lube oil degraderRhodococcussp. 51T7

Abstract
Strain 51T7 identified as a Rhodococcus sp., was isolated from an oil‐contaminated soil sample. When this microorganism was cultivated in a mineral medium with waste lubricant oil as sole carbon substrate, it produced extracellular glycolipids with surface activity. After purification, the main fraction of the surface active compound was identified by 1H NMR and 13C NMR spectroscopy as 2,3,4,2'‐trehalose tetraester. The chain lengths of fatty acids ranged from 7 to 11, and succinoyl residues were detected. Transformation of the waste lube oil was followed by IR spectra.