llβ-Hydroxy dehydroepiandrosterone in a Case of Virilizing Adrenal Adenoma: Isolation from Urine and Mitochondrial Conversion from Dehydroepiandrosterone
- 1 July 1978
- journal article
- research article
- Published by The Endocrine Society in Journal of Clinical Endocrinology & Metabolism
- Vol. 47 (1) , 102-104
- https://doi.org/10.1210/jcem-47-1-102
Abstract
Llβ-Hydroxydehydroepiandrosterone has been isolated from the urine of a 14-yr-old girl with a virilizing adrenal adenoma. Its excretion was estimated to be 0.4 mg/24 h by gas chromatography and the compound was further identified by mass spectrometry. When [7-3H]dehydroepiandrosterone was incubated with mitochondria prepared from the adenoma, approximately 10% was converted to llβ-hydroxydehydroepiandrosterone. The identity of the radioactive β-hydroxydehydroepiandrosternone was verified by reversed isotopic dilution, its conversion to β-hydroxyandrostenedione, and its mobility in several chromatographic systems. This is the first demonstration of an β-hydroxylase from a human source having an affinity for dehydroepiandrosterone.Keywords
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