Abstract
Three paired-feeding experiments were conducted to determine the effect on growth, metabolism, and body composition of rats fed one or five meals daily. Each experiment, with six littermate pairs of castrated male rats, lasted 30 days including an 11-day metabolism trial. Rats fed five meals ("multiple-feeders") gained weight at a faster (P < 0.05) rate and yielded heavier (P < 0.05) dry body weights than did those fed one meal daily ("single-feeders"). A higher percentage of fat in the single-feeders resulted in little difference in the weight of carcass fat. A heavier weight of body protein in the multiple-feeders was consistent with their greater (P < 0.001) retention of nitrogen. Water intake and the urinary losses of water and nitrogen were greater (P < 0.001) for the single-feeders.

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