The Effect of Nutrition on the Sporulation of Melanconium fuligineum in Culture

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Abstract
M. fuligineum is deficient for thiamine; neither growth nor sporulation occurs in the absence of this vitamin. Of the 64 combinations of C and N sources tested, maltose-ammonium tartrate and maltose-casein hydrolysate were superior for rapid and abundant sporulation. Growth and sporulation were poor on media containing either starch or lactose. On glycine sporulation was good but delayed. Rapid and abundant sporulation was correlated with rapid germination of the spores used for inoculum, and rapid early vegetative growth.