Physico-chemical (GC-MS) measurements of juvenile hormone III titres during embryogenesis ofLocusta migratoria

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Abstract
The titre fluctuations of juvenile hormone III (JH III) in eggs of Locusta during embryogenesis were investigated by use of a combination of gas chromatography and chemical ionisation mass spectrometry. The results do not favour the idea of a functional transfer of JH III from the adult female to the embryo. JH III was found to be absent during the period of intense, JH-sensitive, organogenesis, roughly at mid-embryogenesis. The hormone titres peak shortly before the deposition of the last (3rd) embryonic cuticle (which corresponds to the first larval cuticle). Hatching occurs in the virtual absence of JH III.