Rapid preparation of a panel of polyclonal antibodies to Drosophila segmentation proteins
- 1 July 1998
- journal article
- research article
- Published by Springer Nature in Wilhelm Roux' Archiv für Entwicklungsmechanik der Organismen
- Vol. 208 (5) , 290-294
- https://doi.org/10.1007/s004270050184
Abstract
We describe a method for rapidly raising a panel of high quality polyclonal antibodies from bacterially expressed proteins. Approximately 12/3 days of preparation is required per protein. One step that speeds up the procedure is the visualization of purified bands by precipitated sodium dodecyl sulfate (SDS). Antigenicity of the purified recombinant proteins may be increased by precipitation in double-distilled water. The results of using the serums obtained for fluorescent staining of Drosophila embryos are shown.Keywords
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