Increase in cell‐surface N‐acetylglucosaminide β(1→4)galactosyltransferase activity with retinoic acid‐induced differentiation of F9 embryonal carcinoma cells

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Abstract
Exposure of F9 cells to all‐trans‐retinoic acid over a period of 6 days resulted in 4‐fold induction of cell surface N‐acetylglucosaminide β(1→4)galactosyltransferase (GT) activity. The retinoic acid‐induced GT activity was further enhanced by treatment of the cells with 8‐bromo cyclic AMP. The ability of retinoic acid alone, or retinoic acid in combination with 8‐bromo cyclic AMP, to induce GT activity was inhibited by both actinomycin D and cycloheximide. These findings indicate that the induction of galactosyltransferase activity noted with differentiation of F9 cells involves de novo synthesis of new enzyme protein.