Cloning and expression of murine immune interferon cDNA.

Publisher Website
Google Scholar
Abstract
The murine immune interferon (IFN-.gamma.) gene was cloned and expressed under control of the SV40 early promoter in the monkey COS-1 cell line. A protein secreted by these cells had the biological, antigenic and biochemical characteristics of natural murine IFN-.gamma.. Cloned murine IFN-.gamma. cDNA were obtained by using RNA from mitogen-induced murine spleens and the transfected COS cells; both coded for identical proteins. The mature murine IFN-.gamma. encoded was 136 amino acids long, 10 amino acids shorter than human IFN-.gamma.. The nucleotide homology between the murine and human IFN-.gamma. genes was 60-65%; the encoded proteins was only 40% homologous. Murine IFN-.gamma. cDNA was expressed in Escherichia coli under trp promoter control.