Studies on Denitrification: VIII.* Production of Nitric Oxide by Denitrifying Reaction in the Presence of Tetramethyl-p-Phenylenediamine**

1 December 1968

journal article
Published by Oxford University Press (OUP) in The Journal of Biochemistry

Vol. 64 (6) , 849-861
https://doi.org/10.1093/oxfordjournals.jbchem.a128967

Abstract

1. TMPD reduces nitrite to nitric oxide through cytochrome c-552 (denitrifying bacteria) in the presence of denitrifying enzyme. The optimal pH is 6.4–6.6. 2. Carbon monoxide combines with the denitrifying enzyme itself and inhibits its activity; the inhibition is not relieved by light. There occurs a competition for the denitrifying enzyme between CO and NO produced by the denitrification. 3. Nitric oxide production is strongly inhibited by KCN but not by NaN8 in the presence of TMPD. Effects of various other inhibitors are also investigated. 4. By these results the possibility that NO may be an intermediate in the denitrifying reaction was reinforced. 5. A possible scheme for the process of denitrification was proposed.

Keywords

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