Production of Two Lymphocyte Chemotactic Factors by Antigen-Stimulated Guinea Pig Lymphocytes

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Abstract
Lymph node cells from guinea pigs sensitized by BCG elaborated two chemotactic factors for lymphocytes when stimulated with purified protein derivative in vitro 4 weeks after immunization. Production of the factors occurred within 24 h of incubation. The two factors were termed α- and β-lymphocyte chemotactic factors (α-LCF, β-LCF), relative to their elution order on gel chromatography. The molecular weights were about 160,000 and 27,000, respectively. α-LCF was labile to heating at 56 °C for 30 min. Both factors were sensitive to digestion by trypsin, and were effective for T cells but not for B cells.