Preparation and Digestibility of Alfalfa Leaves and Stems Marked with Gold or Chromium

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Abstract
Alfalfa leaves and stems were prepared four ways — unprocessed, water-boiled, acid-boiled and neutral detergent fiber (NDF). Au and Cr were each attached to one set of preparations. Less Au or Cr was attached to NDF (2.53 and 12.6 mg/g dry residue, respectively) than to the other preparations (4.6 to 5.3 mg Au/g dry residue and 17.5 to 27.7 mg Cr/g dry residue). Highest recovery of marker was found with NDF; some marker may have attached to cell solubles in the unprocessed, water-boiled and acid-boiled preparations. Marked leaves and stems had lower in vitro digestibilities than unmarked; attachment of Cr reduced in vitro digestion more than did attachment of Au, except in NDF. Marker concentrations appeared adequate for detection in ingesta or feces when added to the diet in small amounts. Copyright © 1981. American Society of Animal Science . Copyright 1981 by American Society of Animal Science.

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