Comparison of slide agglutination test and direct immunofluorescence assay for identification of Legionella isolates

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Abstract
The results obtained with the direct immunofluorescence assay were compared with the results of a simple and less demanding slide agglutination test for identifying 15 serogroups representing 7 Legionella spp. The slide agglutination test was in complete agreement with the direct immunofluorescence assay; the serogroup to which 64 clinical isolates of Legionella belonged was correctly identified. With polyvalent, pooled antisera and absorbed, serogroup-specific antisera, the slide agglutionation test is a useful alternative to the direct immunofluorescence assay in the diagnosis of Legionella infections and for studying the serological relationships of Legionella-like organisms.