Determination of Alpha-Tocopherol Levels in Rat Microsomes by High-Performance Liquid Chromatography
- 1 October 1988
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 11 (14) , 2977-2982
- https://doi.org/10.1080/01483918808076773
Abstract
A rapid, simple and sensitive method is described for the routine determination of alphatocopherol in microsomes. After a single extraction step with n-heptane extracts are injected onto a normal phase column using n-heptane/isopropanol (99.3/0.7, v/v) for elution; a fluorescence detector set at 215 nm excitation wavelenght and a cut-off filter emitting at 320 nm are used for detection. Total run time is less than 4 minutes.This publication has 15 references indexed in Scilit:
- Simultaneous measurement of tocopherols and tocopheryl quinones in tissue fractions using high-performance liquid chromatography with redox-cycling electrochemical detectionJournal of Chromatography B: Biomedical Sciences and Applications, 1987
- Determination of vitamin E in different biological samples by high-pressure liquid chromatographyAnalytical and Bioanalytical Chemistry, 1986
- Simultaneous determination of serum vitamin A and E by liquid chromatography with fluorescence detectionJournal of Chromatography B: Biomedical Sciences and Applications, 1985
- The monoenoic fatty acid composition of a marine species ofDesulfobulbus grown on lactateLipids, 1985
- Effect of vitamin E on the balance between pro- and antioxidant activity of ascorbic acid in microsomes from rat heart, kidney and liverToxicology Letters, 1985
- Influence of vitamin E and selenium on glutathione-dependent protection against microsomal lipid peroxidationBiochemical Pharmacology, 1984
- Determination of Alpha-Tocopherol in Biological Specimens by High-Performance Liquid ChromatographyJournal of Liquid Chromatography, 1983
- Is vitamin E the only lipid-soluble, chain-breaking antioxidant in human blood plasma and erythrocyte membranes?Archives of Biochemistry and Biophysics, 1983
- Determination of vitamin e in foods — a reviewC R C Critical Reviews in Food Science and Nutrition, 1980
- Isolation and comparison of endoplasmic reticulum membranes and their mixed function oxidase activities from mammalian extrahepatic tissuesPharmacology & Therapeutics, 1979