Differential Expression of the Two Arabidopsis Nitrate Reductase Genes
- 1 May 1991
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 96 (1) , 275-279
- https://doi.org/10.1104/pp.96.1.275
Abstract
The differential regulation of the two nitrate reductase (NR, EC 1.6.6.1) genes of Arabidopsis thaliana L. Heynh was examined. cDNAs corresponding to each of the NR genes (NR1 and NR2) were used to measure changes in the steady-state levels of NR mRNA in response to nitrate, light, circadian rhythm, and tissue specificity. Although nitrate-induction kinetics of the two genes are very similar, NR1 is expressed in the absence of nitrate at a higher basal level than NR2. Nitrate induction is transient both in the roots and leaves, however the kinetics are different: the induction and decline in the roots precede that in the leaves. Light induces the expression of each of the genes with significantly different kinetics: NR2 reached saturation more rapidly than did NR1. Both genes showed similar diurnal patterns of circadian rhythm, with NR2 mRNA accumulating earlier in the morning.Keywords
This publication has 14 references indexed in Scilit:
- Isolation of Transcriptionally Regulated Root-Specific Genes from TobaccoPlant Physiology, 1990
- Intact Plastids Are Required for Nitrate- and Light-Induced Accumulation of Nitrate Reductase Activity and mRNA in Squash CotyledonsPlant Physiology, 1990
- cDNA Clones for Corn Leaf NADH:Nitrate Reductase and Chloroplast NAD(P)+:Glyceraldehyde-3-Phosphate DehydrogenasePlant Physiology, 1989
- Photosynthetic Genes of Petunia (Mitchell) Are Differentially Expressed during the Diurnal CyclePlant Physiology, 1989
- Diurnal and Circadian Rhythms in the Accumulation and Synthesis of mRNA for the Light-Harvesting Chlorophyll a/b-Binding Protein in TobaccoPlant Physiology, 1988
- Expression of Leaf Nitrate Reductase Genes from Tomato and Tobacco in Relation to Light-Dark Regimes and Nitrate SupplyPlant Physiology, 1988
- Phytochrome-Mediated Light Regulation of Nitrate Reductase Expression in Squash CotyledonsPlant Physiology, 1988
- Sequence and nitrate regulation of the Arabidopsis thaliana mRNA encoding nitrate reductase, a metalloflavoprotein with three functional domains.Proceedings of the National Academy of Sciences, 1988
- Genomic sequencing.Proceedings of the National Academy of Sciences, 1984
- A technique for radiolabeling DNA restriction endonuclease fragments to high specific activityAnalytical Biochemistry, 1983