Free Radical-induced Cross-linking of Polydeoxythymidylic Acid in Deoxygenated Aqueous Solution

Abstract
Radiation-generated hydroxyl radicals and hydrogen atoms were shown to induce the cross-linking of polydeoxythymidylic acid (mol. wt .apprx. 170000) in N2O-saturated aqueous solution. The irradiated samples were hydrolysed with formic acid and then analysed by high performance liquid chromatography. Products were isolated and subsequently characterized by capillary gas chromatography-mass spectrometry. The presence of previously described monomeric thymine products was also shown. Yields were determined and mechanisms of formation were described for the products.

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