A two-dimensional YAC pooling strategy for library screeningviaSTS andAlu-PCR methods
Open Access
- 25 May 1992
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 20 (10) , 2559-2563
- https://doi.org/10.1093/nar/20.10.2559
Abstract
We have developed a simple two-dimensional YAC pooling strategy to facilitate YAC library screening via STS and Alu-PCR approaches. The method has been implemented using the human total genomic YAC library of Olson and coworkers, and its validity tested by isolation of many chromosomes 19- and 21-specific YACs. The Alu-PCR approach is notable in that it is hybridization-based, such that PCR primer pairs do not need to be repeatedly synthesized and tested for each screening step.Keywords
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