UV‐transparent, replaceable agarose gels for molecular‐sieve (capillary) electrophoresis of proteins and nucleic acids

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Abstract
Gels of methoxylated agarose (gelling point 25.6 °C) and other low‐melting agarose derivatives compare favorably with cross‐linked polyacrylamide gels for capillary and slab molecular‐sieve electrophoresis of proteins and DNA. These agarose gels can be pressed out of the capillary following a run and replaced by an agarose solution with a temperature of 35–40 °C. Gelation occurs upon lowering the temperature and the same capillary can thus be reused for another analysis with a fresh gel. The methoxylated, non UV‐absorbing agarose gels are, accordingly, replaceable, which makes them very attractive for series analyses with modern, automated capillary electrophoresis apparatus. The high resolution of these agarose gels is demonstrated with a separation of an albumin sample into monomers, dimers, trimers, tetramers, pentamers, hexamers, heptamers, and of DNA fragments.