Tumorigenicity of human lymphoblastoid cell lines, acquired during in vitro culture and associated with chromosome gains

Publisher Website
Google Scholar
Abstract
Tumorigenicity of human lymphoma and lymphoblastoid B‐cell lines was assessed by their ability to form growing and transplantable masses on subcutaneous inoculation into neonatally thymectomized, Ara‐C‐protected, totalbody‐irradiated mice. By these criteria, 12 lines of known malignant origin were tumorigenic, 11 lymphoblastoid lines, tested after less than one year of in vitro growth, were non‐tumorigenic and 8/18 long‐established lymphoblastoid lines produced transplantable tumours. All of the long‐established lines had acquired karyotypic changes on prolonged culture, the predominant characteristic being a gain of whole chromosomes or of major chromosome segments. None showed the classical 8:14 translocation associated with Burkitt's lymphoma. Comparisons with nontumorigenic precursors (recovered from liquid nitrogen storage) and with other non‐tumorigenic but chromosomally abnormal, lymphoblastoid lines suggest that imbalance of the dosage of genes carried on chromosomes 7,8 and 9 may be important in determining the tumorigenic phenotype.

This publication has 33 references indexed in Scilit: