Inhibitory action of galactose on phagocytes from normal and hypergalactosemic chicks

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Abstract
The inhibitory effect of galactose on phagocyte function was investigated in normal and hypergalactosemic chicks by monitoring the in vitro killing of Escherichia coli by leukocytes and the in vivo clearance of colloidal 125I-labeled bovine serum albumin ([125I]BSA) from the circulation. Elevated levels of galactose (30 mM) significantly impaired the bactericidal activities of leukocytes from control and hypergalactosemic chicks. The latter cells were more susceptible to the galactose-dependent inhibition. Leukocytes from hypergalactosemic chicks displayed near-normal bactericidal activity when assayed in vitro under simulated normal conditions in the absence of galactose. Mean corrected phagocytic indexes, obtained from data on the clearance of colloidal [125I]BSA, were calculated to be 0.358 and 0.299 for control and hypergalactosemic chicks, respectively. Increased concentrations of galactose significantly impaired the bactericidal activity of circulating leukocytes but did not significantly affect the phagocytic activity of the RES. [This study was a model for phagocyte function in galactosemic humans.].