Human but not ovine isolates ofBordetella parapertussis are highly clonal as determined by PCR-based RAPD fingerpringting
- 1 September 1998
- journal article
- research article
- Published by Springer Nature in Infection
- Vol. 26 (5) , 270-273
- https://doi.org/10.1007/bf02962245
Abstract
The DNA fingerprints of 170 human isolates and ten ovine isolates ofBordetella parapertussis were examined by arbitrarily-primed PCR/RAPD with 29 primers. Based on this technique, all the human isolates appear highly genetically homogenous. The ovine isolates could be distinguished from human isolates and they showed diversity among themselves. Therefore, human isolates ofB. parapertussis are a highly clonal group adapted to infect humans and they are distinct from polymorphic ovine isolates.Keywords
This publication has 19 references indexed in Scilit:
- Characterization of Bordetella Strains and Related Bacteria by Amplified Ribosomal DNA Restriction Analysis and Randomly and Repetitive Element-Primed PCRInternational Journal of Systematic and Evolutionary Microbiology, 1997
- The Differentiation of Bordetella parapertussis and Bordetella bronchiseptica from Humans and Animals as Determined by DNA Polymorphism Mediated by Two Different Insertion Sequence Elements Suggests Their Phylogenetic RelationshipInternational Journal of Systematic and Evolutionary Microbiology, 1996
- Differentiation between human and ovine isolates ofBordetellaparapertussisusing pulsed-field gel electrophoresisFEMS Microbiology Letters, 1996
- Use of Arbitrarily Primed Polymerase Chain Reaction Analysis to Type Disease and Carrier Strains of Neisseria meningitidis Isolated during a University OutbreakThe Journal of Infectious Diseases, 1994
- Isolation and characterization of Bordetella parapertussis-like bacteria from ovine lungsMicrobiology, 1994
- RAPD (arbitrary primer) PCR is more sensitive than multilocus enzyme electrophoresis for distinguishing related bacterial strainsNucleic Acids Research, 1993
- DNA polymorphisms amplified by arbitrary primers are useful as genetic markersNucleic Acids Research, 1990
- Fingerprinting genomes using PCR with arbitrary primersNucleic Acids Research, 1990
- Bordetella parapertussisfrom lambsNew Zealand Veterinary Journal, 1987
- Virulence Factors of Bordetella PertussisAnnual Review of Microbiology, 1986