The regeneration of plants from frozen pollen embryos and zygotic embryos of wheat and rice
- 1 April 1984
- journal article
- research article
- Published by Springer Nature in Theoretical and Applied Genetics
- Vol. 67 (6) , 525-528
- https://doi.org/10.1007/bf00264898
Abstract
Anther culture derived pollen embryos and immature zygotic embryos of wheat and rice, frozen in liquid nitrogen in the presence of dimethyl sulfoxide, sucrose and glycerol, have been revived. The retrieved cultures proliferated and/or regenerated shoots and plantlets. The prospects of the cryopreservation of embryos for the conservation and multiplication of germplasm and the possibility of the establishment of ‘Germplasm Banks’ are discussed.This publication has 5 references indexed in Scilit:
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