Characterization of Borrelia burgdorferi Isolated from Erythema Migrans Lesions: Interrelationship of Three Molecular Typing Methods
Open Access
- 1 August 2001
- journal article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 39 (8) , 2954-2957
- https://doi.org/10.1128/jcm.39.8.2954-2957.2001
Abstract
Genetic diversity among Borrelia burgdorferi isolates recovered from the skin of Lyme disease patients was assessed by ribosomal DNA (rDNA) spacer restriction fragment length polymorphism analysis, genomic restriction site polymorphism analysis, and plasmid content analysis. There was a significant association between the three rDNA spacer types, the six pulsed-field gel types, and plasmid content ( P < 0.001). The association between distinct chromosomal and plasmid markers implies a clonal origin for each genotype.Keywords
This publication has 31 references indexed in Scilit:
- Association of Specific Subtypes ofBorrelia burgdorferiwith Hematogenous Dissemination in Early Lyme DiseaseThe Journal of Infectious Diseases, 1999
- Evidence for frequent OspC gene transfer betweenBorrelia valaisianasp. nov. and other Lyme disease spirochetesFEMS Microbiology Letters, 1999
- Phylogenetic Analysis of Borrelia burgdorferi Sensu Stricto by Arbitrarily Primed PCR and Pulsed-Field Gel ElectrophoresisInternational Journal of Systematic and Evolutionary Microbiology, 1997
- Genetic Heterogeneity of Borrelia burgdorferi in the United StatesThe Journal of Infectious Diseases, 1997
- The clinical spectrum of early lyme borreliosis in patients with culture-confirmed erythema migransThe American Journal of Medicine, 1996
- Emerging Bacterial Zoonotic and Vector-Borne DiseasesPublished by American Medical Association (AMA) ,1996
- Evidence for lateral transfer and recombination in OspC variation in Lyme disease BorreliaMolecular Microbiology, 1995
- Borrelia burgdorferi is clonal: implications for taxonomy and vaccine development.Proceedings of the National Academy of Sciences, 1993
- Delineation of Borrelia burgdorferi Sensu Stricto, Borrelia garinii sp. nov., and Group VS461 Associated with Lyme BorreliosisInternational Journal of Systematic and Evolutionary Microbiology, 1992
- Analysis of supercoiled circular plasmids in infectious and non-infectious Borrelia burgdorferiMicrobial Pathogenesis, 1990