Comparison of Various Physical Means of Liberation of the Agglutinogen from H. Pertussis in Phase I
Open Access
- 1 October 1940
- journal article
- Published by Oxford University Press (OUP) in The Journal of Immunology
- Vol. 39 (4) , 287-295
- https://doi.org/10.4049/jimmunol.39.4.287
Abstract
Summary: The sonic method of cellular disintegration is the most effective of the six physical means tried for liberation of the phase I-agglutinogen of H. pertussis. The low temperature ball-mill is next and offers more effective means of obtaining the agglutinogen than grinding the dried organisms at room temperature. Semi-quantitative agglutinin-absorption was used as the method of biological assay. Soluble nitrogen content of the extracts does not afford a satisfactory criterion of relative efficiency of extraction of surface-antigen.Keywords
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