glnD and mviN Are Genes of an Essential Operon in Sinorhizobium meliloti
- 15 April 2001
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 183 (8) , 2682-2685
- https://doi.org/10.1128/jb.183.8.2682-2685.2001
Abstract
To evaluate the role of uridylyl-transferase, the Sinorhizobium meliloti glnD gene was isolated by heterologous complementation in Azotobacter vinelandii . The glnD gene is cotranscribed with a gene homologous to Salmonella mviN . glnD1 ::Ω or mviN1 ::Ω mutants could not be isolated by a powerful sucrose counterselection procedure unless a complementing cosmid was provided, indicating that glnD and mviN are members of an indispensable operon in S. meliloti .Keywords
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