X-Ray Structure of T4 Endonuclease V: an Excision Repair Enzyme Specific for a Pyrimidine Dimer
- 24 April 1992
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 256 (5056) , 523-526
- https://doi.org/10.1126/science.1575827
Abstract
The x-ray structure of T4 endonuclease V, an enzyme responsible for the first step of a pyrimidine-dimer-specific excision-repair pathway, was determined at a 1.6-angstrom resolution. The enzyme consists of a single compact domain classified into an all-alpha structure. This single domain has two distinct catalytic activities; it functions as a pyrimidine dimer glycosylase and as an apurinic-apyrimidinic endonuclease. The amino-terminal segment penetrates between two major helices and prevents their direct contact. The refined structure suggests the residues involved in the substrate binding and the catalysis of the glycosylation reaction.Keywords
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