UPTAKE OF I 131 BY GRAM POSITIVE AND GRAM NEGATIVE BACTERIA

Publisher Website
Google Scholar
Abstract
Fixed smears were subjected to the Gram stain procedure with radioactive iodine (I131) incorporated in Gram''s iodine. The uptake and retention of radioactive iodine was measured before and after decolorization and after counterstaining. Crystal violet-stained Gram-positive organisms took up only slightly more I131 as compared with Gram-negative organisms. Gram-positive cells retained significantly more I131 than Gram-negative cells after decolorization and counterstaining. Varying the cell concentration or I131 concentration did not alter this pattern. No significant differences in uptake of I131 were seen between Gram-positive and Gram-negative organisms in the unstained state.