Electrophoresis studies of human synovial mucin

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Abstract
Human synovial mucin was prepared from normal, post-mortem and pathological fluids, including many from patients with rheumatoid arthritis. Electrophoretic studies at pH 8.6 show that the main constituents are albumin and a fast-moving component. The fast-moving component seems to be hyaluronic acid but might be the complex described by Ogston and Stanier (1950). Particularly in clots from arthritic fluids, [alpha]- and [beta]-globulins may be present, though in small amounts. No evidence was obtained for gamma-globulins. Analysis of the electrophoretic patterns indicated the presence of 15-18% of hyaluronic acid in a few clots from normal and pathological fluids. This apparent constancy and the fact that only slight changes were caused by reprecipitation suggest a constant combining ratio of hyaluronic acid and protein.