transActivity of the Norovirus Camberwell Proteinase and Cleavage of the N-Terminal Protein Encoded by ORF1
Open Access
- 15 June 2003
- journal article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 77 (12) , 7150-7155
- https://doi.org/10.1128/jvi.77.12.7150-7155.2003
Abstract
The virus-encoded proteinase of Camberwell virus, a genogroup 2 norovirus, was synthesized inEscherichia coli. The purified proteinase had correct N and C termini and showedtransactivity in cell-free assays.transactivity was also demonstrated in COS cells transfected with constructs encoding either the proteinase or a proteinase-polymerase fusion. The N-terminal protein of ORF1 was cleaved in COS cells, possibly at the site E194/S.Keywords
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