Fyn binding protein, Fyb, interacts with mammalian actin binding protein, mAbp1
- 25 March 2005
- journal article
- Published by Wiley in FEBS Letters
- Vol. 579 (11) , 2339-2347
- https://doi.org/10.1016/j.febslet.2005.03.031
Abstract
The immune cell specific protein Fyn-T binding protein (Fyb) has been identified as a target of the Yersinia antiphagocytic effector Yersinia outer protein H (YopH), but its role in macrophages is unknown. By using Fyb domains as bait to screen a mouse lymphoma cDNA library, we identified a novel interaction partner, mammalian actin binding protein 1 (mAbp1). We show that mAbp1 binds the Fyb N-terminal via its C-terminally located src homology 3 domain. The interaction between Fyb and mAbp1 is detected in macrophage lysates and the proteins co-localize with F-actin in the leading edge. Hence, mAbp1 is likely to constitute a downstream effector of Fyb involved in F-actin dynamics.Keywords
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