Some Characteristics of Nitrate Reductase From Higher Plants
Open Access
- 1 June 1968
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 43 (6) , 930-940
- https://doi.org/10.1104/pp.43.6.930
Abstract
With respect to cofactor requirements, NADH, and FMNH2 were equally effective as electron donors for nitrate reductase obtained from leaves of maize, marrow, and spinach, when the cofactors were supplied in optimal concentrations. The concentration of FMNH2 required to obtain half-maximal activity was from 40- to 100-fold higher than for NADH. For maximal activity with the corn enzyme, 0.8 millimolar FMNH2 was required. In contrast, NADPH was functional only when supplied with NADP:reductase and exogenous FMN (enzymatic generation of FMNH2).Keywords
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