Chloroplast-Diphenyl Ether Interactions II

Abstract
Acifluorfen, a p-nitrodiphenyl ether herbicide, is inhibitory to those photosynthetic functions that require a functioning chloroplast envelope. Functions involving the stroma are also affected. Acifluorfen does not lyse intact spinach chloroplasts, yet does increase the sensitivity of CO2-dependent O2 evolution to exogenous inorganic PO4-3 without directly affecting the function of the PO4-3 translocator. Acifluorfen penetrates into the chloroplast stroma in a light-independent fashion. Once inside, it causes the inactivation of light and dithiothreitol-activated fructose, 1,6-bisphosphatase. Light-activated glyceraldehyde-3-phosphate dehydrogenase (NADP) is also inactivated by acifluorfen. Acifluorfen may stimulate a pathway for inactivation of fructose 1,6-bisphosphatase and glyceraldehyde 3-phosphate dehydrogenase (NADP) which uses O as a terminal oxidant and which involves thioredoxin and ferredoxin-thioredoxin reductase.

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