Use of microcarrier culture for the production of herpes simplex virus (type 2) in MRC‐5 cells

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Abstract
The ultimate aim of this work is the production of a subunit vaccine of herpes simplex virus (HSV). The first step was towards the development of a high‐yielding process for the production of HSV in tissue cells. The microcarrier system was evaluated for this purpose as it is a unit process with good scale‐up potential and is a monolayer‐growth method, essential for HSV production. Details are given of a closed perfusion system with environmental control which was capable of growing MRC‐5 cells to densities in excess of 105 cm−2 (3 × 106 cm−3). However, it has proved difficult, so far, to obtain good titres of HSV in this system. A novel approach to the production of HSV antigen with cell membrane‐associated antigens was tried. Preliminary trials in a guinea‐pig model indicated that these membrane‐associated antigens afforded protection against genital infection with HSV‐2.

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