Quantitative Detection of Protein Binding onto DNA by Using a Quartz-Crystal Microbalance

Abstract
Selective binding process of proteins to DNA strands immobilized on an Au electrode of a quartz-crystal microbalance (QCM) was observed in situ from frequency decrease (mass increase). The binding kinetics of sequence specific binding of NFI to DNA containing its recognition site were compared with those of non-specific binding of histone f3.