The β subunit of human rod photoreceptor cGMP‐gated cation channel is generated from a complex transcription unit
- 1 July 1996
- journal article
- Published by Wiley in FEBS Letters
- Vol. 389 (2) , 213-218
- https://doi.org/10.1016/0014-5793(96)00588-1
Abstract
Human and bovine rod photoreceptor cGMP‐gated cation channel consists of two subunits: α (63 kDa) and β (240 kDa). The human β subunit was shown to consist partly of sequence encoded by the cDNA clone hRCNC2b. Here we present the complete sequence of the human β subunit and demonstrate that the previously reported human GAR1 gene encoding a glutamate‐rich protein (hGARP) encodes its N‐terminal portion. Using PCR, RNA blot and genomic DNA analysis, we provide evidence that the β subunit is produced from a complex locus on chromosome 16 which is also capable of generating independent transcripts corresponding to GAR1 and the C‐terminal two‐thirds of the β subunit. The results indicate that the β subunit of the cGMP‐gated cation channel is produced from an unusual locus consisting of more than one transcription unit.Keywords
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