LOCALIZATION OF HUMAN T-LYMPHOCYTES IN TISSUE-SECTIONS BY A ROSETTING TECHNIQUE

  • 1 January 1977
    • journal article
    • research article
    • Vol. 88  (2) , 323-+
Abstract
A technique for the formation of sheep erythrocyte (E) rosettes in frozen human tissue sections is reported. The labile nature of the receptor for E rosettes on lymphocytes requires the use of controlled conditions for tissue processing and the reaction with indicator cells. the distribution of E rosettes in sections of normal human thymus, lymph nodes, tonsils and spleens was comparable to that of the T [thymus-derived] marker-positive cells identified by immunofluorescence with the specific anti-human T cell serum. There was no overlap with areas positive for 19S EAC [E, antibody, complement] and 7S EA rosettes. Erythocytes treated with a sulfhydryl reagent, 2-aminoethylisothiuronium bromide (AET) and with neuraminidase formed better rosettes in sections than did untreated E. E. rosettes in tissue sections can determine changes in the distribution of T cells in different lymphoproliferative and infiltrating disorders.