Rapid Determination of [Carbon-14] Glucose Specific Radioactivity for In Vivo Glucose Kinetics
Open Access
- 1 June 1975
- journal article
- research article
- Published by American Dairy Science Association in Journal of Dairy Science
- Vol. 58 (6) , 952-956
- https://doi.org/10.3168/jds.s0022-0302(75)84662-5
Abstract
Specific radioactivity of blood glucose was determined by use of a Dowex-1 anion-exchange column after (carbon-14) glucose was infused for in vivo kinetics. The first 20-ml eluate of protein-free blood filtrate from the column was discarded; then 10 ml was collected, lyophilized, and counted in a liquid-scintillation counter. Glucose concentration was determined and specific radioactivity calculated. Kinetic results were comparable to those obtained with the more laborious glucose-pentaacetate procedure.Keywords
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