IN VITRO PRODUCTION OF STEROID CATARACT IN BOVINE LENS

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Abstract
Fresh calf lenses were incubated in nutritive media, to which ouabain and dexamethasone phosphate were added at concentrations of 1 .times. 10-4 M. At the end of a 3-day incubation period, cortical opacification developed in the control series of lenses and those lenses incubated in ouabain and dexamethasone phosphate. Using a light transmission device which quantitates lens opacification, dexamethasone as observed to produce a level of cortical opacification significantly greater than that of the control series. Ouabain produced a level of cortical opacification statistically identical to that produced by the dexamethasone. The aforementioned light transmission device apparently is an accurate and reproducible method of quantitating cataractous opacification.