Isolation of Poliomyelitis Virus from Human Serum by Direct Inoculation into a Laboratory Mouse

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Abstract
Serum and cerebrospinal fluid, from a patient with a probable diagnosis of poliomyelitis, were injected into mice (both brown and white), hamsters and cotton rats. As none of the animals became ill, blind passages were made. One mouse developed paralysis with the second passage strain. A 10% suspension of brain and spinal cord from this animal was injected in 6 brown and 6 white mice. All these mice developed paralysis after 9-26 days. The infection was carried through 22 consecutive passages. Hamsters, cotton rats and guinea pigs were inoculated with the 9th mouse passage strain designated as W.W. The hamsters developed paralysis and the strain was carried through 7 hamster passages. Neutralizing antibodies were developed in monkeys against this W.W. strain and some neutralization was shown against the Lansing and MEF1 strains of poliomyelitis virus. Cross immunization was obtained with the 3 strains. This is the first report of a successful isolation of poliomyelitis virus by direct inoculation into laboratory mice.

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