Binding of aminoacyl-tRNA to rat liver ribosomal proteins
- 1 July 1976
- journal article
- research article
- Published by Springer Nature in Molecular Biology Reports
- Vol. 2 (6) , 471-477
- https://doi.org/10.1007/bf00356936
Abstract
Rat liver ribosome treatment with ethanol and 1 M NH4Cl releases some 31–33 ribosomal proteins. This split protein fraction binds Phe-tRNA, Ac-Phe-tRNA, Met-tRNAM and f-Met-tRNAF in the absence of K+ and Mg++ ions. When the split protein fraction is passed through Sephadex G-100 only six proteins are retained in the column: S10, S14, S15, S19, L35, and L36. The aminoacyl-tRNA binding activity of this protein fraction retained in the Sephadex G-100 column is similar to that of the total split protein fraction, suggesting that the above six proteins, or only some of them, are involved in the binding reaction.This publication has 9 references indexed in Scilit:
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