DNA sequence of theE. coli gyrB gene: application of a new sequencing strategy
Open Access
- 26 January 1987
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 15 (2) , 771-784
- https://doi.org/10.1093/nar/15.2.771
Abstract
We have determined the sequence of the E. coli gyrB gene, using a new sequencing approach in which transposition from a mini-Mu plasmid into the DNA provides random start points for dideoxynucleotide sequence analysis. The gyrB sequence corresponds to a protein 804 amino acids long; a previously isolated protein fragment with partial enzymatic activity has been identified as the C-termlnal half-molecule. A plausible terminator of gyrB transcription is located just beyond the structural gene.Keywords
This publication has 31 references indexed in Scilit:
- MECHANISM OF BACTERIOPHAGE MU TRANSPOSITIONAnnual Review of Genetics, 1986
- Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectorsGene, 1985
- Physical mapping and nucleotide sequence of the rnpA gene that encodes the protein component of ribonuclease P in Escherichia coliGene, 1985
- Mechanism of transposition of bacteriophage mu: polarity of the strand transfer reaction at the initiation of transpositionCell, 1984
- Site-specific recognition of the bacteriophage mu ends by the mu a proteinCell, 1984
- Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesisGene, 1983
- In vitro transposition of bacteriophage Mu: A biochemical approach to a novel replication reactionCell, 1983
- DNA TopoisomerasesAnnual Review of Biochemistry, 1981
- Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencingJournal of Molecular Biology, 1980
- DNA Gyrase and the Supercoiling of DNAScience, 1980