Purification and Immobilization ofAspergillus Niger Pectinase on Magnetic Latex Beads

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Abstract
A commercial preparation of pectinase pectinex was purified with the help of alginate-magnetite beads. The purified pectinase was immobilized an magnetic latex beads via carbodiimide coupling. The maximum efficiency of the immobilized preparation was calculated as 81% of the total activity bound to the beads. This was at the lowest level of coupling tried. The pH optimum (pH 4.5 for both free as well as immobilized enzyme) and km (0.7 mg/ml for free enzyme; 1 mg/ml for immobilized enzyme) did not vary significantly upon immobilization. While the half life of free enzyme was calculated as 9 min., the immobilized preparation remained stable up to 3 h at 60d`C.