Resistance of heat‐shocked cells ofListeria monocytogenesto mano‐sonication and mano‐thermo‐sonication

Abstract
Heat shocks did not increase the resistance of Listeria monocytogenes to an ultrasonication treatment under pressure (Mano-Sonication; MS). While heat-shocked cells (180 min, 45 °C) became sixfold more heat resistant than native cells (D62 = 1·8 min vs D62 = 0·24 min), the resistance of native and heat-shocked cells to MS (200 kPa, 117 μm) was the same (DMS = 1·6 min). The inactivation rate of non-heat-shocked cells of L. monocytogenes by a combined heat/ultrasonication treatment under pressure (Mano-Thermo-Sonication; MTS) was an additive effect. On the contrary, on heat-shocked cells, the inactivation rate of MTS was greater than that of heat added to the inactivation rate of MS (synergistic effect) in the range 62–68 °C.