Abstract
The 13C n.m.r. spectra and incorporations of 13C-labelled precursors into pyrone and dihydroisocoumarin pentaketide metabolites from the culture liquors of Aspergillus melleus are reported. Detection of a two-bond 13C–13C coupling in a metabolite enriched from [1,2-13C2]acetate provides proof for an intramolecular rearrangement occurring during biosynthesis.

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