New map of bacteriophage lambda DNA

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Abstract
A map of bacteriophage .lambda. was constructed, including accurate positions for all 41 cut sites made by 12 different restriction enzymes. Over 100 fragments from single, multiple and partial enzyme digestions were measured vs. standards that were calibrated with respect to DNA molecules of known sequence. The data were subjected to least-squares analysis to assign map coordinates. In no case did a fragment size predicted from the map differ from the measurement of the fragment by more than .+-. 5%. This low error rate was consistent in all size ranges of fragments. The total length of .lambda. was calculated as 49,133 nucleotide pairs. This probably is accurate to within 500 base pairs.