Quantitative imaging for discovery and assembly of the metabo‐regulome

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Abstract
Contents Summary 271 I. Introduction 272 II. From individual enzymes to a complete parts list in 100 yr 272 III. Pathways and flux 273 IV. Quantitative imaging for flux analysis 274 V. Förster resonance energy transfer (FRET) as a tool 275 VI. Methods for FRET determination and analysis of FRET changes 276 VII. FRET sensors for determination of steady‐state concentrations and composite flux, as well as their change 278 VIII. A ‘Gedankenexperiment’ illustrating the potential for flux analysis using FRET sensors 280 IX. The in vivo measurement set‐up 285 X. Spatial resolution of FRET metabolite analyses 286 XI. Potential effect of other parameters on the sensor response 287 XII. Alternatives to FRET sensors for monitoring metabolite concentrations 287 XIII. In vivo analysis of metabolite flux in microorganisms 288 XIV. In vivo analysis of metabolite flux in mammalian cells 288 XV. In vivo analysis of metabolite flux in plants 289 XVI. Application of FRET sensors for high‐throughput analyses 290 Acknowledgements 291 References 291