Immunolocalization of the 100 K nucleolar protein in CHO cells

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Abstract
The localization of a major nucleolar protein with a MW of 100,000 was followed in Chinese hamster ovary cells using specific antibodies to this protein and immunocytochemical techniques. By immunofluorescence and immunocytochemistry at ultrastructural level in situ, the 100 K [kdalton] protein was detected abundantly in the nucleolus of interphase cells. In exponentially growing cells, the dense fibrillar component was shown to contain more 100 K protein than the granular RNP [ribonuclearprotein] component but both the nucleolar components were positively immunostained. Fibrillar centers consistently showed weaker or no staining. The 100 k association with the preribosomal RNP components of the interphase nucleolus supports evidence for a role of this protein in pre-rRNA transcription and preribosomal processing.