Apoptosis of biliary epithelial cells in primary biliary cirrhosis and primary sclerosing cholangitis
- 1 June 2002
- journal article
- research article
- Published by Wiley in Liver International
- Vol. 22 (3) , 228-234
- https://doi.org/10.1046/j.0106-9543.2002.01595.x
Abstract
Background/Aims: Primary biliary cirrhosis (PBC) is an autoimmune disease characterized by inflammatory destruction of small bile ducts. Primary sclerosing cholangitis (PSC) is a different, presumed autoimmune cholestatic liver disease where the bile ducts are also destroyed. In this study, apoptosis and portal triad inflammation in liver tissue from patients with PBC is examined and compared to that from patients with PSC and patients with normal liver.Methods: Explanted liver tissue from patients with PBC and PSC and normal liver from patients with metastases to liver were examined. The liver samples were stained for apoptosis using the terminal deoxynucleotidyl triphosphate (TdT)‐mediated deoxyuridine triphosphate nick end labelling (TUNEL) assay. The biliary epithelial cells (BEC) were then scored on the basis of their TUNEL stain and the degree of periductal inflammation.Results: In PBC, apoptosis of BEC, as detected by the TUNEL assay, was significantly increased in the presence of inflammation. Regardless of the presence or absence of inflammation, the small bile ducts in PBC liver tissue exhibited greater evidence of apoptosis than did similar ducts from PSC or control livers.Conclusion: These findings suggest that in PBC, unlike PSC, the apoptosis of BEC in PBC is secondary to the invasion of inflammatory cells.Keywords
This publication has 14 references indexed in Scilit:
- Expression and cytokine regulation of immune recognition elements by normal human biliary epithelial and established liver cell lines in vitroJournal of Hepatology, 1998
- A novel role for ursodeoxycholic acid in inhibiting apoptosis by modulating mitochondrial membrane perturbation.Journal of Clinical Investigation, 1998
- Enhanced apoptosis relates to bile duct loss in primary biliary cirrhosisHepatology, 1997
- Enhanced apoptosis relates to bile duct loss in primary biliary cirrhosisHepatology, 1997
- A Short-Term Study of Chimeric Monoclonal Antibody cA2 to Tumor Necrosis Factor α for Crohn's DiseaseNew England Journal of Medicine, 1997
- Nuclear DNA fragmentation and expression of Bcl-2 in primary biliary cirrhosisHepatology, 1997
- Apoptosis in the Pathogenesis and Treatment of DiseaseScience, 1995
- Randomised double-blind comparison of chimeric monoclonal antibody to tumour necrosis factor α (cA2) versus placebo in rheumatoid arthritisThe Lancet, 1994
- Electron microscopic observation of destruction of biliary epithelium in primary biliary cirrhosisLiver International, 1983
- Staging of chronic nonsuppurative destructive cholangitis (syndrome of primary biliary cirrhosis)Virchows Archiv, 1978